NanoPhotometer® N50
Nucleic Acid & Protein UV-Assay Measurements
Starting at 0.3 µl | Scan Range 200 – 650 nm
Features
- Detection Range
dsDNA: 5 – 7,500 ng/µl | BSA: 0.15 – 217 mg/ml - Standalone or Remote Operated – Windows, Mac, Android, and iOS
- Lightweight & Portable – Only 20 x 20 x 12 cm footprint
- No maintenance and recalibration required ever
- 5-Star Customer Support – Live application support 24/5
Benefits
- Saves time and avoids manual dilution errors
- Improved sample homogeneity for more accurate readings
- Flexible unit control and access to data from anywhere
- Ready to go into a glove box, under the hood, from lab bench to lab bench, or into the field
- Expert knowledge when you need it
Preprogrammed Methods & Features
- Nucleic Acids (DNA, RNA, mRNA, Oligos, etc.) with QC Ratios (260/280 and 260/230)
- Protein UV with QC Ratio (260/280)
- Measure fluorescent dye-labeled Nucleic Acids and Proteins using absorbance
- Potential contaminant identification
- Further Life Science applications: OD600, Protein Assays (Bradford, BCA, Biuret), Kinetics in a drop
- UV-Vis Spectroscopy applications: Wavelength (including formula editor), Wavescan, Standard Curve, Concentration, Absorbance Ratio
“Best small volume spec on the market”
Rating: 5.0 ★★★★★
“The Implen NanoPhotometer N50 is extremely easy to use (intuitive menus and settings), accurate, and genuinely capable of measuring very small volumes repeatably. The touch screen works well even with gloves. Window configurations are adaptable so you can customize the information you’re looking at while working.
The instrument is light and mobile, and since it’s all-in-one, you can literally move it to where you’re working as needed. It’s not stuck in a particular corner of the lab. In my opinion, it’s the best nano-scale measurement device on the market for routine lab sample quantification and spectral reading.”
David Rawling, Organization: Inflammatix, Inc., USA
N50: Best-in-Class Microvolume Spectrophotometer
Fast & Maintenance-Free N50 Spectrophotometer
Turn on and instantly measure without lamp warm up time. Full scan capability range from 200 – 650 nm for rapid and complete sample analysis in as little as 2.5 seconds. True Path Technology™ covers the extensive dynamic range with two precisely defined path lengths.
The sealed mechanical setup has two fixed anchor points that remain unchanged over the lifetime of the instrument. No recalibration is necessary – Implen guarantees lifetime accuracy.
Compact & Stand-Alone
No computer required. Built-in computer with high-performance Intel Celeron dual-core 2.4 GHz processor and 64 GB of onboard memory provide powerful and easy storage of methods and data.
Preprogrammed UV/Vis spectrophotometer applications are available for nucleic acid quantitation and purity check using the 260/280 and 260/230 ratio (dsDNA, ssDNA, RNA, mRNA, miRNA, oligos including dye-labeled samples) as well as for protein quantitation and purity check using the 260/280 ratio (BSA, antibodies, IgG, IgE, Serum Albumin, etc., including dye-labeled samples), small molecules in organic solvents, kinetics, and more.
Accurate Spectroscopy
Sample Compression Technology™ delivers exceptional precision and accuracy for UV/Vis spectrophotometer measurements, even with challenging samples such as proteins. By creating a stable capillary film between two quartz surfaces, it eliminates dependence on surface tension and prevents sample evaporation, ensuring consistent and reliable measurement conditions.
This innovative technology provides accurate nucleic acid and protein quantification, dependable 260/280 and 260/230 purity assessments, and supports a wide range of UV/Vis spectroscopy applications.
Flexible Unit Control
Mobile and easy control of the NanoPhotometer® N50 is possible via Wi-Fi from tablets or smartphones (Android OS & iOS). Easy control from PCs (Windows & Mac) is also available, with fast and flexible connection options including Wi-Fi, USB, and LAN.
Mobile Design
Microvolume spectroscopy can now be taken anywhere. This compact, lightweight UV/Vis spectrophotometer measures just 20 × 20 × 12 cm and weighs between 3.8 and 5.2 kg, depending on the configuration, making it ideal for portable and field-based applications.
Endless Connectivity
Equipped with Wi-Fi, built-in HotSpot, LAN, HDMI, and USB A/B interface connections for remote instrument control, data accessibility, and printing. NPOS now supports AirPrint/IPP-compatible printers for PDFs, printers compatible with the HP universal driver, and DYMO label printers (cryo and standard labels). The NanoPhotometer® N50 is compatible with 1D and 2D barcode readers, allowing for flexible and streamlined data entry. Unique file server functionality allows access of data stored on the NanoPhotometer® drive from any computer accessing the lab network.
Certainty in Real Time
Sample Control™ is an advanced quality control technology for the N120 UV/Vis spectrophotometer. It helps identify air bubbles, sample impurities, turbidity, lint residue, and potential contamination while also supporting 260/280 and 260/230 ratio calculations.
By monitoring handling characteristics and sample quality in real time, Sample Control™ helps ensure highly precise, reproducible concentration measurements for applications such as nucleic acid and protein quantification.
Universal Data Output
Every NanoPhotometer® is able to generate formatted Excel and PDF document files for data processing or archiving of measurements on the lab network. The capability to print full page reports, labels, and cryo labels via USB and through a network connection lends users the utmost flexibility for data output. Supported printers include AirPrint/IPP-compatible printers for PDFs, printers with HP universal printer driver capability, and DYMO label printers.
AirPrint® is a registered trademark of Apple Inc. Cupertino, CA
Ultimate Data Security
Malware, viruses, and ransomware are serious threats to instrumentation operating on Android- or Windows-based platforms. The proprietary NanoPhotometer® Operating System NPOS protects data in a secure environment immune to known malicious software.
Whether running apps on the NanoPhotometer®/control device or downloading precious measurement results, your data is safe. For additional security the NanoPhotometer® does not allow browsing on the internet whilst providing safe access to control devices like computers, tablets, and smartphones.
Confidence with Blank Control™
Be sure that your data are precise with Implen’s unique Blank Control™ feature for UV/Vis spectrophotometers. Blanks with high background or residue from previous samples are a major source of inaccurate readings. Blank Control™ prevents you from wasting time and precious samples on inaccurate readings caused by high background blanks or improper cleaning.
Universal Language Support
The NPOS interface is offered in a selection of languages. The advanced language functionality allows the operator to effectively understand and interact with the unit, boosting productivity in the lab. In addition to a multilingual user interface, the NanoPhotometer® provides the flexibility to print results in multiple languages, which prevents misinterpretation of data and time-consuming translations.
The selection covers most widely spoken languages including English, Chinese, French, German, Japanese, Portuguese, Russian, and Spanish, and will be continuously extended.
NanoPhotometer® N50 Tech Specs
Microvolume Spectrophotometer | 0.3 µl | Scan Range 200 – 650 nm
General Specifications
Dimensions: 20 cm x 20 cm x 12 cm
Weight: 3.8 – 5.2 kg depending on configuration
Processing Power/Software
- Linux based NPOS
- Intel Celeron dual core 2.4 GHz
- 64 GB Internal Data Storage
- 2x USB A, USB B
- HDMI, Ethernet, WiFi
- Windows 8, 10, 11 (32 & 64 bit)
- OS X (Intel x86 and Apple M1)
- iOS and Android OS
Display
- 1024 x 600 pixels
- Glove compatible touchscreen
NanoVolume
Performance
| Detection Range dsDNA | 5 – 7,500 ng/µl |
| Detection Range BSA | 0.15 – 217 mg/ml |
| Minimum Sample Size | 0.3 – 2 µl |
| Photometric Range (10 mm equivalent) | 0.1 – 150 A |
| Path Length | 0.67 and 0.07 mm |
| Dilution Factor | 15 and 140 |
Optical
Specifications
| Wavelength Scan Range | 200 – 650 nm |
| Measure Time For Full Scan Range | 2.5 – 4 seconds |
| Wavelength Reproducibility | ± 1 nm |
| Wavelength Accuracy | 1.5 nm |
| Bandwidth | < 3 nm |
| Absorbance Reproducibility | (Lid 15): < 0.004 A @ 0 – 0.3 A @ 280 nm CV < 1% @ 0.3 – 1.5 A @ 280 nm |
| Absorbance Accuracy | < 1.75% @ 0.7 A @ 280 nm of the reading |
| Stray Light | < 2% @ 240 nm using NaI |
| Optical Arrangement | 1 x 4096 CMOS Array |
| Lamp | Xenon flash lamp |
| Lifetime | 109 flashes, up to 10 years |
General
Specifications
| Operating Voltage | 90 – 250 V ± 10%, 50/60 Hz, 90 W, 18/19 VDC |
| Certification | CE, IEC 61010-1:2012 and EN 61326-1:2013 |
| Security | Slot for Kensington lock |