Limitless Spectroscopy Built-in Apps

Empowering Every Field of Research

Dive into a world of possibilities with Implen’s UV/Vis spectrophotometers — designed to accommodate a full spectrum of lab applications, from nucleic acids and proteins to cell density and kinetic studies. Whether you’re measuring tiny DNA samples, quantifying proteins, monitoring bacterial growth, or conducting absorbance scans across 200–900 nm, our instruments bring precision and versatility to every workflow.

Explore how the NanoPhotometer® can transform your research with flexible, high-accuracy, maintenance-free spectroscopy solutions.

Nucleic Acids

Fast DNA and RNA Quantification

The Nucleic Acids method enables fast quantification and purity analysis of dsDNA, ssDNA, RNA, mRNA, miRNA, and oligonucleotides using microvolume UV-Vis spectroscopy.

The NanoPhotometer automatically calculates concentration along with the 260/280 and 260/230 purity ratios to assess sample quality. The method also supports analysis of dye-labeled nucleic acids by determining dye concentration and labeling efficiency. Results are displayed with a full absorbance spectrum and a table of raw data points for detailed evaluation.

Protein UV

Direct A280 Protein Analysis

The Protein UV method enables rapid protein quantification using direct A280 absorbance measurements. It supports common proteins such as antibodies, IgG, IgE, serum albumin, and other purified proteins. Concentrations can be calculated using the extinction coefficient for the selected protein or reported as OD values.

The method also supports dye-labeled proteins with built-in and customizable dye settings to determine labeling efficiency. Each measurement includes a full spectrum scan and a detailed table of raw absorbance data.

Protein Assays

Colorimetric Protein Quantification

The Protein Assays method allows accurate protein quantification using common colorimetric protein assays. Supported assays include Bradford, BCA, Lowry, and Biuret, enabling flexible analysis across different protein concentrations and sample types.

Standard curves can be generated directly within the NanoPhotometer software for precise concentration determination. Results are displayed with graphical plots and complete measurement data, making the method suitable for routine protein analysis in molecular biology and biochemical laboratories.

OD600 Cell Density

Cell Density Measurement

The OD600 method measures optical density at 600 nm to determine bacterial cell density in liquid culture. This measurement is widely used to monitor microbial growth and determine the optimal time for induction, harvesting, or downstream experiments.

A linear relationship exists between cell density and OD600 within the typical growth range, allowing reliable estimation of culture concentration. The method provides fast, repeatable measurements suitable for microbiology, biotechnology, and fermentation workflows.

McFarland Method

Standardized Bacterial Turbidity

The McFarland method enables standardized measurement of bacterial suspension turbidity according to McFarland standards, commonly used in microbiology laboratories. It supports preparation and verification of bacterial concentrations for applications such as antimicrobial susceptibility testing and culture standardization. The Implen OD600 compares sample turbidity to McFarland reference values using precise optical measurements, ensuring consistent and reproducible results for clinical and research workflows.

Kinetics

The Kinetics method measures changes in absorbance over time at a fixed wavelength using either microvolume samples or cuvettes. It is commonly used for enzyme kinetics and reaction monitoring in food, beverage, and clinical laboratories. The NanoPhotometer displays absorbance values, ΔA, slope, and correlation coefficient for accurate kinetic analysis.

Wavescan

The Wavescan method records full absorption or transmission spectra between two user-defined wavelengths. This allows easy identification of peak height and position across the spectrum. Spectral scanning provides detailed information about sample composition and optical properties.

Wavelength

The Wavelength method measures absorbance or transmission at a single user-defined wavelength. This simple measurement provides a quick evaluation of sample optical properties relative to a reference. It is commonly used for routine spectrophotometric measurements.

Concentration

The Concentration method determines sample concentration from a single wavelength absorbance reading. A user-defined factor or standard can be used to convert absorbance into concentration values. This approach enables quick quantification for many spectrophotometric assays.

Absorbance Ratio

The Absorbance Ratio method calculates the ratio between two user-defined wavelengths. Ratio measurements are commonly used to evaluate sample purity, composition, or relative concentration. The method provides a fast way to compare spectral features of a sample.

Standard Curve

The Standard Curve method generates calibration curves using standards of known concentration. Unknown samples can then be quantified based on the established curve. The NanoPhotometer allows curves to be saved and reused for future experiments.

Custom Apps

The Custom Apps option enables development of fully customized applications using the NanoPhotometer® operating system. Users can design specialized methods tailored to unique workflows or analytical requirements. Contact the Implen team for more information on custom application development.