NanoPhotometer® NP80
Microvolume and Cuvette Functionality | Built-in Vortex
Starting at 0.3 µl | Scan Range 200 – 900 nm | 2.5 sec
Features
- Broad Detection Range*
dsDNA: 1 – 16,500 ng/µl | BSA: 0.03 – 478 mg/ml - Bandwidth better than 1.5 nm
- Robust instrument design without the need for routine calibration checks
- Standalone or Remote Operated – Windows, Mac, Android, and iOS
- Portable with Battery Option – Only 20 x 20 x 12 cm footprint
- 5-Star Customer Support – Live application support 24/5
- GMP, GLP, GxP Ready 21 CFR Part 11 and IQ/OQ option*
Benefits
- Saves time and avoids manual dilution errors
- Ready for pharmaceutical applications and full dynamic range (e.g., for OD600 readings)
- Zero cost of ownership
- Flexible unit control and access to data from anywhere
- Ready to go into a glove box, under the hood, from lab bench to lab bench, or into the field
- Expert knowledge when you need it
- Full laboratory compliance with peace of mind
*Cuvette detection range dsDNA: 0.1 – 130 ng/µl, BSA: 0.003 – 3.7 mg/ml
Preprogrammed Methods & Features
- Nucleic Acids (DNA, RNA, mRNA, Oligos, etc.) with QC Ratios (260/280 and 260/230)
- Protein UV with QC Ratio (260/280)
- Measure fluorescent dye-labeled Nucleic Acids and Proteins using absorbance
- Potential contaminant identification
- Further Life Science applications: OD600, Protein Assays (Bradford, BCA, Biuret), Kinetics in a drop
- UV-Vis Spectroscopy applications: Wavelength (including formula editor), Wavescan, Standard Curve, Concentration, Absorbance Ratio
“Great results and very accurate!”
Rating: 5.0 ★★★★★
“I love love love this machine. It’s portable, idiot proof, and accurate. For its DNA analysis, it is much more accurate than the old familiar… I love the fact that it is so modifiable and easy to use. We use it for a variety of functions in the lab, including Bradford assays. I really love that there is a built-in graph for these and that you can email it to yourself or save on a USB stick.
This machine is the thing we have all been needing but never knew we missed. Also the customer care is outstanding and I look forward to our rep every time she comes.
Andrea Kuipers, Organization: California Institute of Technology, USA
NP80: Best-in-Class Microvolume and Cuvette Spectrophotometer
This lightweight, portable UV/Vis spectrophotometer delivers best-in-class microvolume spectroscopy and cuvette-based analysis for nucleic acid quantification, protein analysis, and a wide range of UV/Vis spectroscopy applications. The integrated 7-inch glove-compatible touchscreen and flexible control options provide an intuitive user experience in both laboratory and field environments.
A built-in vortex mixer ensures sample homogeneity for highly accurate and reproducible results, while requiring as little as 0.3 µL of sample. Implen Load Assist™ uses an integrated red guidance light to make sample drops and air bubbles immediately visible on the measurement surface, helping users confirm proper sample placement before measurement.
Fast & Maintenance-Free UV/Vis NP80 Spectrophotometer
Turn on and instantly measure without lamp warm-up time. Full scan capability range from 200-900 nm for rapid and complete sample analysis in as little as 2.5 seconds. True Path Technology™ covers the extensive dynamic range with two precisely defined path lengths.
The sealed mechanical setup has two fixed anchor points that remain unchanged over the lifetime of the instrument. No recalibration is necessary – Implen guarantees lifetime accuracy.
Compact & Stand-Alone
No computer required. NanoPhotometer’s NP80 built-in computer with high-performance Intel Celeron dual-core 2.4 GHz processor and 64 GB of onboard memory provide powerful and easy storage of methods and data.
Preprogrammed UV/Vis spectrophotometer applications are available for nucleic acid quantitation and purity check using the 260/280 and 260/230 ratio (dsDNA, ssDNA, RNA, mRNA, miRNA, oligos including dye-labeled samples), as well as for protein quantitation and purity check using the 260/280 ratio (BSA, antibodies, IgG, IgE, Serum Albumin, etc., including dye-labeled samples), small molecules in organic solvents, kinetics, and more.
Built-in Cuvette Port
Perfected for OD600 and protein assay (Bradford, BCA) measurements. The built-in high-performance cuvette option (with a spectral resolution < 1.5 nm) in the NanoPhotometer® NP80 provides a dynamic range of up to 2.6 OD, which can be expanded using our proprietary DiluCell™ 10. The DiluCell™ provides an automatic dilution of 1/10, expanding the range to 26 OD (1 cm).
Built-in Vortex Mixer
Achieve sample uniformity by vortexing prior to each measurement with the proprietary built-in NanoPhotometer® NP80 low-vibration/low-energy vortexer for gentle mixing of even delicate samples; obtain readings that represent the accurate concentration of the entire sample.
Mobile Design
Take microvolume spectroscopy wherever your work takes you. The NP80 UV/Vis spectrophotometer features an ultra-compact 20 × 20 × 12 cm footprint and weighs just 3.8 to 5 kg, depending on the configuration, making it one of the most portable instruments in its class.
For maximum flexibility, an optional built-in battery pack provides up to 8 hours of standalone operation, enabling reliable UV/Vis spectroscopy measurements in the laboratory, at the bench, or in the field.
Flexible Unit Control
Mobile and easy control of the NanoPhotometer® is possible via Wi-Fi from tablets or smartphones (Android OS & iOS). Easy control from PCs (Windows & Mac) is also available, with fast and flexible connection options including Wi-Fi, USB and LAN.
Certainty in Real Time
Sample Control™ is an advanced quality control technology for UV/Vis spectrophotometers that automatically detects air bubbles, sample impurities, turbidity, lint residue, and potential contamination. It also supports real-time sample quality assessment through 260/280 and 260/230 ratio measurements, helping ensure reliable nucleic acid and protein analysis.
By continuously monitoring sample integrity and handling characteristics during UV/Vis spectroscopy measurements, Sample Control™ improves data quality, reproducibility, and confidence in concentration results, delivering the highest level of accuracy for microvolume and cuvette-based applications.
Confidence with Blank Control™
Be sure that your data are precise with Implen’s unique Blank Control™ feature for UV/Vis spectrophotometers. Blanks with high background or residue from previous samples are a major source of measurement inaccuracies. Blank Control™ prevents you from wasting time and precious samples on inaccurate readings caused by high background blanks or improper cleaning.
Accurate Spectroscopy
Sample Compression Technology™ delivers exceptional precision and accuracy for UV/Vis spectrophotometer measurements, even with challenging samples such as proteins. By creating a stable capillary film between two quartz surfaces, it eliminates dependence on surface tension and prevents sample evaporation, ensuring consistent and reliable measurement conditions.
This innovative technology provides accurate nucleic acid and protein quantification, dependable 260/280 and 260/230 purity assessments, and supports a wide range of UV/Vis spectroscopy applications.
Endless Connectivity
Equipped with Wi-Fi, built-in HotSpot, LAN, HDMI, and USB A/B interface connections for remote instrument control, data accessibility, and printing. NPOS supports AirPrint/IPP-compatible printers for PDFs, printers compatible with the HP universal driver, and DYMO label printers (cryo and standard labels).
The NanoPhotometer® NP80 is compatible with 1D and 2D barcode readers allowing for flexible and streamlined data entry. Unique file server functionality allows access of data stored on the NanoPhotometer® drive from any computer accessing the lab network.
Ultimate Data Security
Malware, viruses, and ransomware are serious threats to instrumentation working on Android- or Windows-based platforms. The proprietary NanoPhotometer® Operating System NPOS protects data in a secure environment immune to known malicious software.
Whether running apps on the NanoPhotometer®/control device or downloading precious measurement results, your data is safe. For additional security the NanoPhotometer® does not allow browsing on the internet whilst providing safe access to control devices like computers, tablets, and smartphones.
Universal Data Output
Every NanoPhotometer® is able to generate formatted Excel and PDF document files for data processing or archiving of measurements on the lab network. The capability to print full page reports, labels, and cryo labels via USB and through a network connection lends users the utmost flexibility for data output. Supported printers include AirPrint/IPP-compatible printers for PDFs, printers with HP universal printer driver capability, and DYMO label printers.
AirPrint® is a registered trademark of Apple Inc. Cupertino, CA
Universal Language Support
The NPOS user interface is offered in a selection of languages. The advanced language functionality allows the operator to effectively understand and interact with the unit, boosting productivity in the lab. In addition to a multilingual user interface, the NanoPhotometer® provides the flexibility to print results in multiple languages, which prevents misinterpretation of data and time-consuming translations.
The selection covers most widely spoken languages including English, Chinese, French, German, Japanese, Portuguese, Russian, and Spanish, and will be continuously extended.
CFR21 Software
The Implen CFR21 software supports FDA 21 CFR Part 11 compliance and is an optional software tool ideal for GxP laboratories, which require proper electronic record-keeping for UV/Vis spectroscopic measurements utilizing a microvolume or cuvette UV/Vis spectrophotometer. It includes user management, access control, electronic signatures, data integrity, security, and audit trail functionality.
Fun for Spectroscopy Nerds: The NanoPhotometer® Crew
Get to know the NanoPhotometer® Crew – the dedicated team behind reliable UV/Vis spectrophotometry for researchers worldwide. Follow their entertaining adventures as they work tirelessly to deliver accurate nucleic acid and protein quantification, dependable purity assessments (260/280 and 260/230 ratios), and precise results across a wide range of UV/Vis applications.
Their mission has been captured on video and is available directly on your instrument through the screensaver feature, included with firmware version 4.3 and later. Activate the screensaver and enjoy a behind-the-scenes look at the crew’s ongoing commitment to scientific excellence.
NanoPhotometer® NP80 Tech Specs
Microvolume, Cuvette, Vortex | 0.3 µl | Scan Range 200 – 900 nm
General Specifications
Dimensions: 20 cm x 20 cm x 12 cm
Weight: 3.8 – 5.2 kg depending on configuration
Processing Power/Software
- Linux based NPOS
- Intel Celeron dual core 2.4 GHz
- 64 GB Internal Data Storage
- 2x USB A, USB B
- HDMI, Ethernet, WiFi
- Windows 8, 10, 11 (32 & 64 bit)
- OS X (Intel x86 and Apple M1)
- iOS and Android OS
Display
- 1024 x 600 pixels
- Glove compatible touchscreen
Built-in Battery Pack
Optional rechargeable lithium ion battery:
95 Wh, 6.6 Ah, 8 h
Min. charging cycles: 800
NanoVolume
Performance
| Detection Range dsDNA | 1 – 16,500 ng/µl |
| Detection Range BSA | 0.03 – 478 mg/ml |
| Minimum Sample Size | 0.3 – 2 µl |
| Photometric Range (10 mm equivalent) | 0.02 – 330 A |
| Path Length | 0.67 and 0.07 mm |
| Dilution Factor | 15 and 140 |
| Vortex | 2,800 rpm; tube size up to 2.0 ml |
Cuvette
Performance
| Detection Range dsDNA | 0.1 – 130 ng/µl |
| Detection Range BSA | 0.003 – 3.7 mg/ml |
| Photometric Range | 0 – 2.6 A |
| Center Height (Z-Height) | 8.5 mm |
| Cell Types | Outside dimension 12.5 x 12.5 mm |
| Heating | 37°C ± 0.5°C |
Optical
Specifications
| Wavelength Scan Range | 200 – 900 nm |
| Measure Time For Full Scan Range | 2.5 – 4 seconds |
| Wavelength Reproducibility | ± 0.2 nm |
| Wavelength Accuracy | ± 0.75 nm |
| Bandwidth | < 1.5 nm |
| Absorbance Reproducibility | (Cuvette): < 0.002 A @ 0 – 0.3 A @ 280 nm CV < 1% @ 0.3 – 2.0 A @ 280 nm (Lid 15): < 0.002 A @ 0 – 0.3 A @ 280 nm CV < 1% @ 0.3 – 1.7 A @ 280 nm |
| Absorbance Accuracy | < 1.75% @ 0.7 A @ 280 nm of the reading |
| Stray Light | < 0.5% @ 240 nm using NaI |
| Optical Arrangement | 1 x 4096 CMOS Array |
| Lamp | Xenon flash lamp |
| Lifetime | 109 flashes, up to 10 years |
General
Specifications
| Operating Voltage | 90 – 250 V ± 10%, 50/60 Hz, 90 W, 18/19 VDC |
| Certification | CE, IEC 61010-1:2012 and EN 61326-1:2013 |
| Battery Certification | IEC 62133 and UN38.3 transport test |
| Security | Slot for Kensington lock |
